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lymphoprep density gradient centrifugation  (Nycomed)

 
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    Nycomed lymphoprep density gradient centrifugation
    Lymphoprep Density Gradient Centrifugation, supplied by Nycomed, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lymphoprep+density+gradient+centrifugation/density+gradient+centrifugation+lymphoprep/us12358989-633-8-12
    Average 90 stars, based on 1 article reviews
    lymphoprep density gradient centrifugation - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Gradient Centrifugation:

    Article Title: Role of HDL in cholesteryl ester metabolism of lipopolysaccharide-activated P388D1 macrophages
    Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were separated by Lymphoprep (Nycomed Pharma AS, Oslo, Norway) density gradient centrifugation, as previously described ( 36 ). ..

    Article Title: Inflammatory Response of Monocytes to Ambient Particles Varies by Highway Proximity
    Article Snippet: .. Isolation of Peripheral Blood Mononuclear Cells and Selection of Peripheral Blood Monocytes Because the THP-1 cells were not differentiated in culture to adherent macrophages, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were also challenged with PM to assess the consistency of response in an ex vivo model. PBMCs were isolated from heparinized peripheral blood using Lymphoprep density gradient centrifugation (Nycomed Pharma AS, Oslo, Norway) and subsequent negative selection using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, Grand Island, NY). ..

    Article Title: Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses
    Article Snippet: .. Human peripheral blood mononuclear cells (PBMC) were isolated from Buffy coats by Lymphoprep density gradient centrifugation (Axis‐shield, Dundee, United Kingdom and Nycomed, Zurich, Switzerland). .. PBMC were cultured in RPMI 1640 supplemented with Glutamax and sodium pyruvate (Gibco), 2.5% FCS and 100 IU/ml penicillin and 100 mg/ml streptomycin (Gibco).

    Article Title: Different perforin expression in peripheral blood and prostate tissue in patients with benign prostatic hyperplasia and prostate cancer.
    Article Snippet: .. Peripheral blood mononuclear cells were isolated by Lymphoprep (Nycomed Pharma AS, Oslo, Norway) density gradient centrifugation (20 min, 600 g). .. Following centrifugation, cells from the interface were collected, washed in Roswell Park Memorial Institute (RPMI) 1640 medium (Invitrogen, Auckland, NZ, USA) and used immediately for further experimental procedures.

    Article Title: Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination
    Article Snippet: .. The cells were purified from whole blood by LymphoPrep density gradient centrifugation (Nycomed, Oslo, Norway). .. mRNA was isolated from the hybridomas HKB1 and L243 by use of a Dynabeads mRNA Direct kit (Dynal, Oslo, Norway), and cDNA was synthesized using a cDNA Synthesis Kit (Amersham Biosciences, Oslo, Norway).

    Article Title: Inflammatory Response of Monocytes to Ambient Particles Varies by Highway Proximity
    Article Snippet: .. Because the THP-1 cells were not differentiated in culture to adherent macrophages, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were also challenged with PM to assess the consistency of response in an ex vivo model. PBMCs were isolated from heparinized peripheral blood using Lymphoprep density gradient centrifugation (Nycomed Pharma AS, Oslo, Norway) and subsequent negative selection using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, Grand Island, NY). ..

    Article Title: High Monocyte Count and Expression of S100A9 and S100A12 in Peripheral Blood Mononuclear Cells Are Associated with Poor Outcome in Patients with Metastatic Prostate Cancer
    Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from the remaining blood sample by Lymphoprep density gradient centrifugation (Nycomed). .. The PBMCs were washed in PBS and 7 × 10 6 cells were used for flow cytometry analyses (BD LSRII, BD Biosciences, Franklin Lakes, NJ, USA) and the remaining PBMCs were frozen in RNA-later (Qiagen) for RT-PCR analysis.

    Article Title: HLA binding vaccine moieties and uses thereof
    Article Snippet: .. The cells were purified from whole blood by LymphoPrep density gradient centrifugation (Nycomed, Oslo, Norway). .. Construction of Vaccine Molecules mRNA was isolated from the hybridomas HKB1 and L243 by use of Dynabeads® mRNA DIRECTTM kit (Dynal, Oslo, Norway), and cDNA synthesized using cDNA Synthesis Kit (Amersham Biosciences, Oslo, Norway).

    Isolation:

    Article Title: Inflammatory Response of Monocytes to Ambient Particles Varies by Highway Proximity
    Article Snippet: .. Isolation of Peripheral Blood Mononuclear Cells and Selection of Peripheral Blood Monocytes Because the THP-1 cells were not differentiated in culture to adherent macrophages, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were also challenged with PM to assess the consistency of response in an ex vivo model. PBMCs were isolated from heparinized peripheral blood using Lymphoprep density gradient centrifugation (Nycomed Pharma AS, Oslo, Norway) and subsequent negative selection using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, Grand Island, NY). ..

    Article Title: Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses
    Article Snippet: .. Human peripheral blood mononuclear cells (PBMC) were isolated from Buffy coats by Lymphoprep density gradient centrifugation (Axis‐shield, Dundee, United Kingdom and Nycomed, Zurich, Switzerland). .. PBMC were cultured in RPMI 1640 supplemented with Glutamax and sodium pyruvate (Gibco), 2.5% FCS and 100 IU/ml penicillin and 100 mg/ml streptomycin (Gibco).

    Article Title: Different perforin expression in peripheral blood and prostate tissue in patients with benign prostatic hyperplasia and prostate cancer.
    Article Snippet: .. Peripheral blood mononuclear cells were isolated by Lymphoprep (Nycomed Pharma AS, Oslo, Norway) density gradient centrifugation (20 min, 600 g). .. Following centrifugation, cells from the interface were collected, washed in Roswell Park Memorial Institute (RPMI) 1640 medium (Invitrogen, Auckland, NZ, USA) and used immediately for further experimental procedures.

    Article Title: Inflammatory Response of Monocytes to Ambient Particles Varies by Highway Proximity
    Article Snippet: .. Because the THP-1 cells were not differentiated in culture to adherent macrophages, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were also challenged with PM to assess the consistency of response in an ex vivo model. PBMCs were isolated from heparinized peripheral blood using Lymphoprep density gradient centrifugation (Nycomed Pharma AS, Oslo, Norway) and subsequent negative selection using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, Grand Island, NY). ..

    Article Title: High Monocyte Count and Expression of S100A9 and S100A12 in Peripheral Blood Mononuclear Cells Are Associated with Poor Outcome in Patients with Metastatic Prostate Cancer
    Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from the remaining blood sample by Lymphoprep density gradient centrifugation (Nycomed). .. The PBMCs were washed in PBS and 7 × 10 6 cells were used for flow cytometry analyses (BD LSRII, BD Biosciences, Franklin Lakes, NJ, USA) and the remaining PBMCs were frozen in RNA-later (Qiagen) for RT-PCR analysis.

    Selection:

    Article Title: Inflammatory Response of Monocytes to Ambient Particles Varies by Highway Proximity
    Article Snippet: .. Isolation of Peripheral Blood Mononuclear Cells and Selection of Peripheral Blood Monocytes Because the THP-1 cells were not differentiated in culture to adherent macrophages, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were also challenged with PM to assess the consistency of response in an ex vivo model. PBMCs were isolated from heparinized peripheral blood using Lymphoprep density gradient centrifugation (Nycomed Pharma AS, Oslo, Norway) and subsequent negative selection using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, Grand Island, NY). ..

    Article Title: Inflammatory Response of Monocytes to Ambient Particles Varies by Highway Proximity
    Article Snippet: .. Because the THP-1 cells were not differentiated in culture to adherent macrophages, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were also challenged with PM to assess the consistency of response in an ex vivo model. PBMCs were isolated from heparinized peripheral blood using Lymphoprep density gradient centrifugation (Nycomed Pharma AS, Oslo, Norway) and subsequent negative selection using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, Grand Island, NY). ..

    Ex Vivo:

    Article Title: Inflammatory Response of Monocytes to Ambient Particles Varies by Highway Proximity
    Article Snippet: .. Isolation of Peripheral Blood Mononuclear Cells and Selection of Peripheral Blood Monocytes Because the THP-1 cells were not differentiated in culture to adherent macrophages, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were also challenged with PM to assess the consistency of response in an ex vivo model. PBMCs were isolated from heparinized peripheral blood using Lymphoprep density gradient centrifugation (Nycomed Pharma AS, Oslo, Norway) and subsequent negative selection using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, Grand Island, NY). ..

    Article Title: Inflammatory Response of Monocytes to Ambient Particles Varies by Highway Proximity
    Article Snippet: .. Because the THP-1 cells were not differentiated in culture to adherent macrophages, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were also challenged with PM to assess the consistency of response in an ex vivo model. PBMCs were isolated from heparinized peripheral blood using Lymphoprep density gradient centrifugation (Nycomed Pharma AS, Oslo, Norway) and subsequent negative selection using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, Grand Island, NY). ..

    Purification:

    Article Title: Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination
    Article Snippet: .. The cells were purified from whole blood by LymphoPrep density gradient centrifugation (Nycomed, Oslo, Norway). .. mRNA was isolated from the hybridomas HKB1 and L243 by use of a Dynabeads mRNA Direct kit (Dynal, Oslo, Norway), and cDNA was synthesized using a cDNA Synthesis Kit (Amersham Biosciences, Oslo, Norway).

    Article Title: HLA binding vaccine moieties and uses thereof
    Article Snippet: .. The cells were purified from whole blood by LymphoPrep density gradient centrifugation (Nycomed, Oslo, Norway). .. Construction of Vaccine Molecules mRNA was isolated from the hybridomas HKB1 and L243 by use of Dynabeads® mRNA DIRECTTM kit (Dynal, Oslo, Norway), and cDNA synthesized using cDNA Synthesis Kit (Amersham Biosciences, Oslo, Norway).



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